Tritrichomonas foetus, a sexually transmitted disease in cattle, continues to plague the cattle industry despite years of control programs. One reason for this continued prevalence throughÂout the U.S. and the world is that accurate diagnosis is compliÂcated in the fact that retrieval of organisms for diagnosis and subsequent testing may be compromised by multiple factors inÂcluding, but not limited to, how samples are collected. A commonly used sampling technique for diagnosing T. foetus in bulls is scraping of the preputial and penile mucosa with eiÂther an artificial insemination pipette or specially designed T. foetus testing device such as the Pizzle Stick Trich Testing DeÂvice (Lane Manufacturing Denver, CO). Parker et al. noted that right-hand dominant practitioners were more commonly sucÂcessful in retrieving T. foetus in known positive bulls when colÂlecting from the right side of the bull compared to the left; howÂever, the opposite was not investigated for left-hand dominant practitioners and only cultures were used to declare whether a sample was positive or not. The objective of this study was to determine if using current RT-qPCR testing if there is a differÂence in rate of positive tests pending side of chute and use of dominant hand for testing.